Hotspot

rps16-trnQ-UUG

LSC · 6145-7256 · 1112 bp

Back to Avicennia

Pi 0.0289

MarkerSeek score 65.40

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1112
variable_sites: 32
pi: 0.0289
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GGATCCTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	57.7	58.1	1199-1212 bp	1.0	79.4
rps16-trnQ-UUG_p2	GATCCTGTCCTTCAAGTCGC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1198-1211 bp	1.0	79.0
rps16-trnQ-UUG_p3	GCGTTGCTTTCTACCACATC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.5	58.1	1178-1191 bp	1.0	77.6
rps16-trnQ-UUG_p4	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.2	58.1	1177-1190 bp	1.0	75.5
rps16-trnQ-UUG_p5	GGATCCTGTCCTTCAAGTCG	TTCGGAGGTTCGAATCCTTC	55.0	50.0	57.7	57.3	1203-1216 bp	1.0	74.6