Hotspot

ccsA-ndhD

SSC · 117473-117733 · 261 bp

Back to Averrhoa

Pi 0.0588

MarkerSeek score 76.40

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 261
variable_sites: 15
pi: 0.0588
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ATCCTGCAATTGTGGCTTCT	CAGTTGACAAGGTCGAAGCT	45.0	50.0	57.8	58.1	412-418 bp	1.0	81.2
ccsA-ndhD_p2	ATCCTGCAATTGTGGCTTCT	TCAGTTGACAAGGTCGAAGC	45.0	50.0	57.8	58.1	413-419 bp	1.0	81.1
ccsA-ndhD_p3	TCCTGCAATTGTGGCTTCTA	CAGTTGACAAGGTCGAAGCT	45.0	50.0	57.1	58.1	411-417 bp	1.0	76.0
ccsA-ndhD_p4	TCCTGCAATTGTGGCTTCTA	TCAGTTGACAAGGTCGAAGC	45.0	50.0	57.1	58.1	412-418 bp	1.0	76.0
ccsA-ndhD_p5	AATCCTGCAATTGTGGCTTC	CAGTTGACAAGGTCGAAGCT	45.0	50.0	57.0	58.1	413-419 bp	1.0	74.6