Hotspot

trnP-UGG-psaJ

LSC · 69269-69743 · 475 bp

Back to Averrhoa

Pi 0.0273

MarkerSeek score 74.20

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 475
variable_sites: 12
pi: 0.0273
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	ACGCATCCGGAAATAAACGA	50.0	45.0	58.1	58.0	603-639 bp	1.0	84.6
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	ACGCATCCGGAAATAAACGA	50.0	45.0	58.1	58.0	602-638 bp	1.0	84.6
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	ACGCATCCGGAAATAAACGA	50.0	45.0	58.1	58.0	614-650 bp	1.0	84.6
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	ACGCATCCGGAAATAAACGA	50.0	45.0	58.1	58.0	613-649 bp	1.0	84.5
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	ACGCATCCGGAAATAAACGA	50.0	45.0	58.1	58.0	616-652 bp	1.0	84.5