Hotspot

petA-psbJ

LSC · 66317-66854 · 538 bp

Back to Averrhoa

Pi 0.0204

MarkerSeek score 69.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 538
variable_sites: 11
pi: 0.0204
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCGTCTGTTATTTTGGCAC	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.0	57.6	649-668 bp	1.0	78.2
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.0	57.6	683-702 bp	1.0	78.0
petA-psbJ_p3	CATTACGTGTCCAAGGCCTT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	680-699 bp	1.0	76.9
petA-psbJ_p4	GGCGTCTGTTATTTTGGCAC	ACCGGTATTCTTGTGATCGG	50.0	50.0	58.0	57.4	698-717 bp	1.0	76.3
petA-psbJ_p5	GGCGTCTGTTATTTTGGCAC	CCGGTATTCTTGTGATCGGT	50.0	50.0	58.0	57.4	697-716 bp	1.0	76.3