Hotspot

atpB-rbcL

LSC · 57929-58724 · 796 bp

Back to Averrhoa

Pi 0.0127

MarkerSeek score 67.30

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 796
variable_sites: 10
pi: 0.0127
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CATCCAGTACCGGACCAATG	GCCTTGAATCCAACACTTGC	55.0	50.0	58.0	57.9	940-959 bp	1.0	81.0
atpB-rbcL_p2	TGAGCGATACGTCCTAGGTT	GCCTTGAATCCAACACTTGC	50.0	50.0	58.0	57.9	917-936 bp	1.0	81.0
atpB-rbcL_p3	TTGAGCGATACGTCCTAGGT	GCCTTGAATCCAACACTTGC	50.0	50.0	58.0	57.9	918-937 bp	1.0	81.0
atpB-rbcL_p4	CATCCAGTACCGGACCAATG	CCAGCCTTGAATCCAACACT	55.0	50.0	58.0	58.1	943-962 bp	1.0	80.9
atpB-rbcL_p5	TGAGCGATACGTCCTAGGTT	CCAGCCTTGAATCCAACACT	50.0	50.0	58.0	58.1	920-939 bp	1.0	80.9