Hotspot

rpoB-trnC-GCA

LSC · 28170-29393 · 1224 bp

Back to Averrhoa

Pi 0.0238

MarkerSeek score 60.80

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1224
variable_sites: 29
pi: 0.0238
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GGTATTGTCGACATTCCCCC	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.0	58.1	1324-1342 bp	1.0	81.2
rpoB-trnC-GCA_p2	CGACATTCCCCCATTTCCAT	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.9	58.1	1316-1334 bp	1.0	80.9
rpoB-trnC-GCA_p3	TGTCGACATTCCCCCATTTC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.9	58.1	1319-1337 bp	1.0	80.5
rpoB-trnC-GCA_p4	CCATTTCCATCTCCGAGCAT	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.7	58.1	1306-1324 bp	1.0	79.4
rpoB-trnC-GCA_p5	GGTATTGTCGACATTCCCCC	AAAAGGATTTGCAGTCCCCC	55.0	50.0	58.0	58.4	1306-1324 bp	1.0	79.1