Hotspot

rpl23

LSC · 55651-55929 · 279 bp

Back to Avena

Pi 0.00498
MarkerSeek score 26.50
Primer available yes
Label rpl23

Feature Metadata

label_name
rpl23
feature_type
gene
region
LSC
length_bp
279
variable_sites
7
pi
0.00498
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
26.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl23_p1 GCCTGTGTACAAGCTCGTAA GCGAAGTCGTCCTGTTATGT 50.0 50.0 57.9 58.0 1275-1282 bp 1.0 81.7
rpl23_p2 GTAAGCTAGAAGGGGAACGC GCGAAGTCGTCCTGTTATGT 55.0 50.0 58.1 58.0 1553-1560 bp 1.0 81.4
rpl23_p3 TGCTCGCGGTATCTTTTTCA GCGAAGTCGTCCTGTTATGT 45.0 50.0 57.9 58.0 1477-1484 bp 1.0 81.3
rpl23_p4 TGCCAGCTCTGACTGAAATC GCGAAGTCGTCCTGTTATGT 50.0 50.0 57.9 58.0 1391-1398 bp 1.0 81.3
rpl23_p5 GTAAGCTAGAAGGGGAACGC AAGCATTGTATCGTGGGGTC 55.0 50.0 58.1 58.0 1413-1417 bp 0.777778 71.2