Hotspot

rpl32-trnL-UAG

SSC · 105040-105670 · 631 bp

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Pi 0.0106
MarkerSeek score 44.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
631
variable_sites
27
pi
0.0106
unique_haplotype_count
13
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGGCAACAACAACAAACAA TTCGAACCGAGATGCTTGAG 45.0 50.0 57.9 58.0 728-735 bp 0.888889 76.7
rpl32-trnL-UAG_p2 GGGGCAACAACAACAAACAA GGATTCGAACCGAGATGCTT 45.0 50.0 57.9 58.1 731-738 bp 0.888889 76.2
rpl32-trnL-UAG_p3 GGGGCAACAACAACAAACAA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.9 57.9 701-708 bp 0.888889 75.8
rpl32-trnL-UAG_p4 GGGGCAACAACAACAAACAA AGCAGCGTGTCTACCAATTT 45.0 45.0 57.9 57.8 694-701 bp 0.888889 75.3
rpl32-trnL-UAG_p5 GGGGCAACAACAACAAACAA GCTTGAGCACTGCTTCCTAA 45.0 50.0 57.9 58.2 715-722 bp 0.888889 75.3