Hotspot

ndhF-rpl32

SSC · 104003-104850 · 848 bp

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Pi 0.00559
MarkerSeek score 42.50
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
848
variable_sites
25
pi
0.00559
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
42.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CCCATGCATATTGATATATATGTTCCA TCCAAATATTTTTACGAATACGCT 33.3 29.2 57.4 55.3 820-974 bp 1.0 46.2
ndhF-rpl32_p2 ACCCATGCATATTGATATATATGTTCC TCCAAATATTTTTACGAATACGCT 33.3 29.2 57.4 55.3 821-975 bp 1.0 46.2
ndhF-rpl32_p3 CCCATGCATATTGATATATATGTTCCA TCCAAATATTTTTACGAATACGCTT 33.3 28.0 57.4 56.0 820-974 bp 1.0 46.2
ndhF-rpl32_p4 CCCATGCATATTGATATATATGTTCCA TTCCAAATATTTTTACGAATACGCT 33.3 28.0 57.4 56.0 821-975 bp 1.0 46.2
ndhF-rpl32_p5 ACCCATGCATATTGATATATATGTTCC TCCAAATATTTTTACGAATACGCTT 33.3 28.0 57.4 56.0 821-975 bp 1.0 46.2