Hotspot

petL-petG

LSC · 63171-63346 · 176 bp

Back to Avena

Pi 0.00521
MarkerSeek score 33.60
Primer available yes
Label petL-petG

Feature Metadata

label_name
petL-petG
feature_type
igs
region
LSC
length_bp
176
variable_sites
4
pi
0.00521
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
33.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petL-petG_p1 ACCCCAGCTCTATTTATTGGC TGCAGTCACGAATAATCCCG 47.6 50.0 57.8 58.1 295-297 bp 1.0 71.3
petL-petG_p2 ACCCCAGCTCTATTTATTGGC CAGTCACGAATAATCCCGCT 47.6 50.0 57.8 57.8 293-295 bp 1.0 70.0
petL-petG_p3 CCCCAGCTCTATTTATTGGCT TGCAGTCACGAATAATCCCG 47.6 50.0 57.5 58.1 294-296 bp 1.0 69.0
petL-petG_p4 TGGCTTGAACAAGATACGTCT TGCAGTCACGAATAATCCCG 42.9 50.0 57.3 58.1 278-280 bp 1.0 67.8
petL-petG_p5 CCCCAGCTCTATTTATTGGCT CAGTCACGAATAATCCCGCT 47.6 50.0 57.5 57.8 292-294 bp 1.0 67.7