Hotspot

petA-psbJ

LSC · 60341-61033 · 693 bp

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Pi 0.00561
MarkerSeek score 50.30
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
693
variable_sites
19
pi
0.00561
unique_haplotype_count
12
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GGCCTTTTGTTCTTCTTCGC TCCTCTTTGGCTGATAGGTACT 50.0 45.5 57.9 58.0 890-898 bp 1.0 65.8
petA-psbJ_p2 GGCCTTTTGTTCTTCTTCGC CCTCTTTGGCTGATAGGTACTG 50.0 50.0 57.9 57.9 889-897 bp 1.0 65.3
petA-psbJ_p3 TGTTCTTCTTCGCATCCGTT TCCTCTTTGGCTGATAGGTACT 45.0 45.5 57.8 58.0 883-891 bp 1.0 64.9
petA-psbJ_p4 TTGTTCTTCTTCGCATCCGT TCCTCTTTGGCTGATAGGTACT 45.0 45.5 57.8 58.0 884-892 bp 1.0 64.9
petA-psbJ_p5 TGTTCTTCTTCGCATCCGTT CCTCTTTGGCTGATAGGTACTG 45.0 50.0 57.8 57.9 882-890 bp 1.0 64.4