Hotspot

psaA-ycf3

LSC · 40725-41367 · 643 bp

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Pi 0.00371
MarkerSeek score 48.20
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
643
variable_sites
15
pi
0.00371
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 GGTCTGGCCCATTCTTCAAA GCGCTTACTCCGGGAAATTA 50.0 50.0 58.1 58.1 800-819 bp 1.0 80.5
psaA-ycf3_p2 GGTCTGGCCCATTCTTCAAA TGGTTTGATCAAGCTGCTGA 50.0 45.0 58.1 57.7 839-858 bp 1.0 78.7
psaA-ycf3_p3 GGTCTGGCCCATTCTTCAAA GGTTGAAGATTACGAAGCGC 50.0 50.0 58.1 57.6 763-782 bp 1.0 77.6
psaA-ycf3_p4 TGTTCTTGAGAAATGGCCGG GCGCTTACTCCGGGAAATTA 50.0 50.0 58.5 58.1 819-838 bp 1.0 77.4
psaA-ycf3_p5 TTTTACTTCTGGTTCCGGCG GCGCTTACTCCGGGAAATTA 50.0 50.0 58.5 58.1 747-766 bp 1.0 77.4