Hotspot

trnR-UCU-trnfM

LSC · 35428-35541 · 114 bp

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Pi 0.0121
MarkerSeek score 46.40
Primer available yes
Label trnR-UCU-trnfM

Feature Metadata

label_name
trnR-UCU-trnfM
feature_type
igs
region
LSC
length_bp
114
variable_sites
7
pi
0.0121
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-trnfM_p1 AGAAGACCTCTGTCCTATCCA CAGGTTCGATTCCCACTACC 47.6 55.0 56.9 57.7 175-180 bp 1.0 64.3
trnR-UCU-trnfM_p2 AGAAGACCTCTGTCCTATCCA CTTGGTAGCTCACAAGGCTC 47.6 55.0 56.9 58.3 210-215 bp 1.0 64.1
trnR-UCU-trnfM_p3 AGAAGACCTCTGTCCTATCCA GCAGGTTCGATTCCCACTAC 47.6 55.0 56.9 58.3 176-181 bp 1.0 64.0
trnR-UCU-trnfM_p4 AGGTTTAGAAGACCTCTGTCCT CAGGTTCGATTCCCACTACC 45.5 55.0 57.9 57.7 181-186 bp 1.0 63.5
trnR-UCU-trnfM_p5 AGGTTTAGAAGACCTCTGTCCT CTTGGTAGCTCACAAGGCTC 45.5 55.0 57.9 58.3 216-221 bp 1.0 63.4