Hotspot

psaC-ndhD

Genome · 125637-125756 · 120 bp

Back to Austrocallerya

Pi 0.025

MarkerSeek score 66.90

Primer available yes

Label psaC-ndhD

Feature Metadata

label_name: psaC-ndhD
feature_type: igs
region: Genome
length_bp: 120
variable_sites: 3
pi: 0.025
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhD_p1	CAGAGGACTGTGTTGGTTGT	ATTAAGGAACCCCCGGCTAT	50.0	50.0	57.7	58.2	298 bp	1.0	76.8
psaC-ndhD_p2	CAGAGGACTGTGTTGGTTGT	CCCGGCTATTGGCAAAACTA	50.0	50.0	57.7	58.2	287 bp	1.0	76.2
psaC-ndhD_p3	CAGAGGACTGTGTTGGTTGT	AATTAAGGAACCCCCGGCTA	50.0	50.0	57.7	58.4	299 bp	1.0	74.9
psaC-ndhD_p4	CAGAGGACTGTGTTGGTTGT	TTAAGGAACCCCCGGCTATT	50.0	50.0	57.7	58.4	297 bp	1.0	74.9
psaC-ndhD_p5	CAGAGGACTGTGTTGGTTGT	AAAATTAAGGAACCCCCGGC	50.0	50.0	57.7	58.4	301 bp	1.0	74.7