Hotspot

rps11-rpl36

Genome · 82985-83382 · 398 bp

Back to Austrocallerya

Pi 0.0405

MarkerSeek score 65.20

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: Genome
length_bp: 398
variable_sites: 16
pi: 0.0405
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	TTTTGGTATAGGTTTTGCCA	CAGGCGAGGGCGAATTATAG	35.0	55.0	52.9	58.3	480-483 bp	1.0	43.2
rps11-rpl36_p2	TTTTTGGTATAGGTTTTGCCA	CAGGCGAGGGCGAATTATAG	33.3	55.0	53.9	58.3	481-484 bp	1.0	43.2
rps11-rpl36_p3	TTTGGTATAGGTTTTGCCAT	CAGGCGAGGGCGAATTATAG	35.0	55.0	52.7	58.3	479-482 bp	1.0	43.2
rps11-rpl36_p4	TTTTGGTATAGGTTTTGCCAT	CAGGCGAGGGCGAATTATAG	33.3	55.0	53.6	58.3	480-483 bp	1.0	43.2
rps11-rpl36_p5	TTTTGGTATAGGTTTTGCCA	ACAGCAGCTTCTGTTCGTAA	35.0	45.0	52.9	57.5	527-530 bp	1.0	42.9