Hotspot

rpl20-rps12

Genome · 72713-73450 · 738 bp

Back to Austrocallerya

Pi 0.00679

MarkerSeek score 54.20

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: Genome
length_bp: 738
variable_sites: 5
pi: 0.00679
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	GTCTTGAATGAGCTCCGTGA	GTCACAAAATCTCCCGCTCT	50.0	50.0	57.6	57.9	891-892 bp	1.0	76.9
rpl20-rps12_p2	AGTCTTGAATGAGCTCCGTG	GTCACAAAATCTCCCGCTCT	50.0	50.0	57.6	57.9	892-893 bp	1.0	76.8
rpl20-rps12_p3	GTCTTGAATGAGCTCCGTGA	TGTCACAAAATCTCCCGCTC	50.0	50.0	57.6	58.2	892-893 bp	1.0	76.1
rpl20-rps12_p4	AGTCTTGAATGAGCTCCGTG	TGTCACAAAATCTCCCGCTC	50.0	50.0	57.6	58.2	893-894 bp	1.0	76.1
rpl20-rps12_p5	GTCTTGAATGAGCTCCGTGA	AGCCAATCCGAAATGTCACA	50.0	45.0	57.6	57.8	905-906 bp	1.0	76.1