Hotspot

petA-psbJ

Genome · 65966-67077 · 1112 bp

Back to Austrocallerya

Pi 0.00921

MarkerSeek score 66.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: Genome
length_bp: 1112
variable_sites: 10
pi: 0.00921
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGTGTCCAAGGTCTCTTGTT	ACTGGTATTGTCGTGATCGG	50.0	50.0	57.8	57.4	1298-1319 bp	1.0	75.6
petA-psbJ_p2	CGTGTCCAAGGTCTCTTGTT	CTGGTATTGTCGTGATCGGT	50.0	50.0	57.8	57.4	1297-1318 bp	1.0	75.6
petA-psbJ_p3	CGTTACGTGTCCAAGGTCTC	ACTGGTATTGTCGTGATCGG	55.0	50.0	58.3	57.4	1303-1324 bp	1.0	75.1
petA-psbJ_p4	CGTTACGTGTCCAAGGTCTC	CTGGTATTGTCGTGATCGGT	55.0	50.0	58.3	57.4	1302-1323 bp	1.0	75.1
petA-psbJ_p5	GATCCGTTACGTGTCCAAGG	CTGGTATTGTCGTGATCGGT	55.0	50.0	58.4	57.4	1306-1327 bp	1.0	74.6