Hotspot

rps16-accD

Genome · 60056-60805 · 750 bp

Back to Austrocallerya

Pi 0.0133

MarkerSeek score 66.40

Primer available yes

Label rps16-accD

Feature Metadata

label_name: rps16-accD
feature_type: igs
region: Genome
length_bp: 750
variable_sites: 10
pi: 0.0133
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-accD_p1	AACAAGGCGCTCAACCTAC	ACAGCATAGAATTGAACCACCA	52.6	40.9	58.1	58.0	879-880 bp	1.0	56.1
rps16-accD_p2	AACAAGGCGCTCAACCTAC	GAGGTCTTCACTTACACTGGT	52.6	47.6	58.1	57.0	968-969 bp	1.0	56.0
rps16-accD_p3	GAACAAGGCGCTCAACCTAC	ACAGCATAGAATTGAACCACCA	55.0	40.9	59.2	58.0	880-881 bp	1.0	55.0
rps16-accD_p4	GAACAAGGCGCTCAACCTAC	GAGGTCTTCACTTACACTGGT	55.0	47.6	59.2	57.0	969-970 bp	1.0	54.9
rps16-accD_p5	GAACAAGGCGCTCAACCTA	GAGGTCTTCACTTACACTGGT	52.6	47.6	57.8	57.0	969-970 bp	1.0	54.8