Hotspot

psbK-trnQ-UUG

Genome · 57665-58328 · 664 bp

Back to Austrocallerya

Pi 0.0138

MarkerSeek score 63.20

Primer available yes

Label psbK-trnQ-UUG

Feature Metadata

label_name: psbK-trnQ-UUG
feature_type: igs
region: Genome
length_bp: 664
variable_sites: 9
pi: 0.0138
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-trnQ-UUG_p1	CGTAGGCTTCAGGCAATTTC	CGAATAACGGGACCAAAACC	50.0	50.0	57.2	57.1	803-852 bp	1.0	68.2
psbK-trnQ-UUG_p2	GTAGGCTTCAGGCAATTTCG	CGAATAACGGGACCAAAACC	50.0	50.0	57.2	57.1	802-851 bp	1.0	68.2
psbK-trnQ-UUG_p3	TAGGCTTCAGGCAATTTCGC	CGAATAACGGGACCAAAACC	50.0	50.0	58.9	57.1	801-850 bp	1.0	67.6
psbK-trnQ-UUG_p4	GCGTAGGCTTCAGGCAATTT	CGAATAACGGGACCAAAACC	50.0	50.0	59.2	57.1	804-853 bp	1.0	65.4
psbK-trnQ-UUG_p5	GTAGGCTTCAGGCAATTTCG	GAATAACGGGACCAAAACCC	50.0	50.0	57.2	56.7	801-850 bp	1.0	65.0