Hotspot

trnR-UCU-trnG-UCC

Genome · 54674-55023 · 350 bp

Back to Austrocallerya

Pi 0.0349
MarkerSeek score 67.00
Primer available yes
Label trnR-UCU-trnG-UCC

Feature Metadata

label_name
trnR-UCU-trnG-UCC
feature_type
igs
region
Genome
length_bp
350
variable_sites
12
pi
0.0349
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-trnG-UCC_p1 AGAAGACCTCTGTCCTATCCA AGCCTTCCAAGCTAACGATG 47.6 50.0 56.9 58.0 424-430 bp 1.0 66.7
trnR-UCU-trnG-UCC_p2 AGAAGACCTCTGTCCTATCCA ACCCTTAGCCTTCCAAGCTA 47.6 50.0 56.9 58.0 430-436 bp 1.0 66.6
trnR-UCU-trnG-UCC_p3 AGGTTTAGAAGACCTCTGTCCT AGCCTTCCAAGCTAACGATG 45.5 50.0 57.9 58.0 430-436 bp 1.0 66.0
trnR-UCU-trnG-UCC_p4 AGGTTTAGAAGACCTCTGTCCT ACCCTTAGCCTTCCAAGCTA 45.5 50.0 57.9 58.0 436-442 bp 1.0 65.9
trnR-UCU-trnG-UCC_p5 AGAAGACCTCTGTCCTATCCA CTAACGATGCGGGTTCGATT 47.6 50.0 56.9 58.4 413-419 bp 1.0 63.6