Hotspot

atpH-atpF

Genome · 50831-51539 · 709 bp

Back to Austrocallerya

Pi 0.0164

MarkerSeek score 72.80

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: Genome
length_bp: 709
variable_sites: 11
pi: 0.0164
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	CCGGAAGCAGAGGGAAAAAT	GATGGCCAGTAACTCAAGCA	50.0	50.0	58.2	57.9	836-876 bp	1.0	82.6
atpH-atpF_p2	TGGGCTGGTTGTAGCATTAG	GATGGCCAGTAACTCAAGCA	50.0	50.0	57.6	57.9	765-805 bp	1.0	80.9
atpH-atpF_p3	CCGGAAGCAGAGGGAAAAAT	CGGATGGCCAGTAACTCAAG	50.0	55.0	58.2	58.3	838-878 bp	1.0	80.7
atpH-atpF_p4	CCGGAAGCAGAGGGAAAAAT	TGGCCAGTAACTCAAGCAAA	50.0	45.0	58.2	57.6	834-874 bp	1.0	80.6
atpH-atpF_p5	AGCAGAGGGAAAAATACGGG	GATGGCCAGTAACTCAAGCA	50.0	50.0	57.6	57.9	831-871 bp	1.0	80.5