Hotspot

ycf3-psaA

Genome · 19888-20896 · 1009 bp

Back to Austrocallerya

Pi 0.00742

MarkerSeek score 67.40

Primer available yes

Label ycf3-psaA

Feature Metadata

label_name: ycf3-psaA
feature_type: igs
region: Genome
length_bp: 1009
variable_sites: 7
pi: 0.00742
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-psaA_p1	AGCCCTTACCCCTGGTAATT	TGCCCATTCCTCGAAAGAAG	50.0	50.0	58.0	57.9	1099-1157 bp	1.0	83.6
ycf3-psaA_p2	AGCCCTTACCCCTGGTAATT	GCCCATTCCTCGAAAGAAGT	50.0	50.0	58.0	57.9	1098-1156 bp	1.0	83.5
ycf3-psaA_p3	AGCTATAGCCCTTACCCCTG	TGCCCATTCCTCGAAAGAAG	55.0	50.0	58.0	57.9	1105-1163 bp	1.0	83.3
ycf3-psaA_p4	AGCTATAGCCCTTACCCCTG	GCCCATTCCTCGAAAGAAGT	55.0	50.0	58.0	57.9	1104-1162 bp	1.0	83.3
ycf3-psaA_p5	TTGGTTGAAGATCACAGGGC	TGCCCATTCCTCGAAAGAAG	50.0	50.0	58.1	57.9	1063-1121 bp	1.0	82.9