Hotspot

trnL-UAA-trnT-UGU

Genome · 14790-16313 · 1524 bp

Back to Austrocallerya

Pi 0.0145
MarkerSeek score 67.50
Primer available yes
Label trnL-UAA-trnT-UGU

Feature Metadata

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAA-trnT-UGU_p1 ACCAATTTCGCCATATCCCC GCGATGCTCTAACCTCTGAG 50.0 55.0 58.0 57.9 1574-1683 bp 1.0 80.3
trnL-UAA-trnT-UGU_p2 TAGCGTCTACCAATTTCGCC GCGATGCTCTAACCTCTGAG 50.0 55.0 58.1 57.9 1582-1691 bp 1.0 79.8
trnL-UAA-trnT-UGU_p3 ACCAATTTCGCCATATCCCC GGAATCGAACCGATGACCAT 50.0 50.0 58.0 57.8 1606-1715 bp 1.0 79.4
trnL-UAA-trnT-UGU_p4 TAGCGTCTACCAATTTCGCC GGAATCGAACCGATGACCAT 50.0 50.0 58.1 57.8 1614-1723 bp 1.0 78.9
trnL-UAA-trnT-UGU_p5 CCCATTGTGAATTCCAGGGT GCGATGCTCTAACCTCTGAG 50.0 55.0 57.8 57.9 1667-1776 bp 1.0 78.4