Hotspot

trnK-UUU-rbcL

Genome · 4635-5701 · 1067 bp

Back to Austrocallerya

Pi 0.00583

MarkerSeek score 65.50

Primer available yes

Label trnK-UUU-rbcL

Feature Metadata

label_name: trnK-UUU-rbcL
feature_type: igs
region: Genome
length_bp: 1067
variable_sites: 6
pi: 0.00583
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rbcL_p1	TGGAGTCGCACTTAAAAGCC	AGTCCTGAATTAGCTGCTGC	50.0	50.0	58.5	58.0	1162-1190 bp	1.0	78.9
trnK-UUU-rbcL_p2	AACCCTACCGATGGTATGGA	AGTCCTGAATTAGCTGCTGC	50.0	50.0	57.5	58.0	1215-1243 bp	1.0	78.7
trnK-UUU-rbcL_p3	AGTCGTGGTCTTACAAACCC	AGTCCTGAATTAGCTGCTGC	50.0	50.0	57.5	58.0	1230-1258 bp	1.0	78.2
trnK-UUU-rbcL_p4	GTCGTGGTCTTACAAACCCT	AGTCCTGAATTAGCTGCTGC	50.0	50.0	57.5	58.0	1229-1257 bp	1.0	78.2
trnK-UUU-rbcL_p5	AGTCGCACTTAAAAGCCGAG	AGTCCTGAATTAGCTGCTGC	50.0	50.0	58.6	58.0	1159-1187 bp	1.0	78.2