Hotspot

rps19

IRa · 54075-54356 · 282 bp

Back to Australopyrum

Pi NA

MarkerSeek score 30.40

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: IRa
length_bp: 282
variable_sites: 0
pi: NA
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.00
markerseek_score: 30.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	TTCTGACCTCCGTACTTCGA	AGATATTGGGTATTTCTGTTACT	50.0	30.4	57.8	52.6	430 bp	0.5	20.0
rps19_p2	TTCTGACCTCCGTACTTCGA	AAGATATTGGGTATTTCTGTTACT	50.0	29.2	57.8	53.5	431 bp	0.5	20.0
rps19_p3	TTCTGACCTCCGTACTTCGA	AGATATTGGGTATTTCTGTTACTT	50.0	29.2	57.8	53.5	430 bp	0.5	20.0
rps19_p4	TCTGACCTCCGTACTTCGAT	AGATATTGGGTATTTCTGTTACT	50.0	30.4	57.6	52.6	429 bp	0.5	20.0
rps19_p5	CCTCCGTACTTCGATCGAGA	AGATATTGGGTATTTCTGTTACT	55.0	30.4	58.4	52.6	424 bp	0.5	20.0