Hotspot

psaJ-rpl33

LSC · 119298-119740 · 443 bp

Back to Australopyrum

Pi 0.0113

MarkerSeek score 62.40

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 443
variable_sites: 5
pi: 0.0113
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TATCTCTCTGTAGCACCCGT	GAAATCCCCGTCGATTCCTC	50.0	55.0	57.3	58.1	644-646 bp	1.0	74.0
psaJ-rpl33_p2	TATCTCTCTGTAGCACCCGT	GGCACCTTTTCGAACACAAC	50.0	50.0	57.3	58.2	621-623 bp	1.0	73.7
psaJ-rpl33_p3	TATCTCTCTGTAGCACCCGT	CGATTCCTCATTGGCACCTT	50.0	50.0	57.3	58.2	633-635 bp	1.0	73.1
psaJ-rpl33_p4	ATCTCTCTGTAGCACCCGTG	GAAATCCCCGTCGATTCCTC	55.0	55.0	58.9	58.1	643-645 bp	1.0	72.7
psaJ-rpl33_p5	ATCTCTCTGTAGCACCCGTG	GGCACCTTTTCGAACACAAC	55.0	50.0	58.9	58.2	620-622 bp	1.0	72.4