Hotspot

rpl23-psaI

LSC · 110841-111902 · 1062 bp

Back to Australopyrum

Pi 0.0295

MarkerSeek score 67.20

Primer available yes

Label rpl23-psaI

Feature Metadata

label_name: rpl23-psaI
feature_type: igs
region: LSC
length_bp: 1062
variable_sites: 16
pi: 0.0295
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.51
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23-psaI_p1	CCCTTCAACCGGGTTATTCT	GTCATTGCAATTGCCGGAAA	50.0	45.0	57.2	57.9	1183 bp	0.5	53.0
rpl23-psaI_p2	AGGTAGAAGAATAGGGCCCA	GTCATTGCAATTGCCGGAAA	50.0	45.0	57.1	57.9	1242 bp	0.5	52.3
rpl23-psaI_p3	CCCTTCAACCGGGTTATTCT	GAAGTCATTGCAATTGCCGG	50.0	50.0	57.2	58.4	1186 bp	0.5	50.7
rpl23-psaI_p4	AAGGTAGAAGAATAGGGCCCA	GTCATTGCAATTGCCGGAAA	47.6	45.0	57.9	57.9	1243 bp	0.5	50.2
rpl23-psaI_p5	AGGTAGAAGAATAGGGCCCA	GAAGTCATTGCAATTGCCGG	50.0	50.0	57.1	58.4	1245 bp	0.5	49.9