Hotspot

psbK-psbI

LSC · 61314-61718 · 405 bp

Back to Australopyrum

Pi 0.0296

MarkerSeek score 65.50

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 405
variable_sites: 12
pi: 0.0296
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	TTTTTACTCCTCGCGTCCAG	50.0	50.0	57.9	57.9	549-554 bp	1.0	80.7
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TTCCGTCCTGGGTCATTAGA	50.0	50.0	57.9	57.8	527-532 bp	1.0	79.4
psbK-psbI_p3	TGGCAAGCTGCTGTAAGTTT	TTTTTACTCCTCGCGTCCAG	45.0	50.0	58.3	57.9	546-551 bp	1.0	78.7
psbK-psbI_p4	TTGGCAAGCTGCTGTAAGTT	TTTTTACTCCTCGCGTCCAG	45.0	50.0	58.3	57.9	547-552 bp	1.0	78.7
psbK-psbI_p5	TTTGGCAAGCTGCTGTAAGT	TTTTTACTCCTCGCGTCCAG	45.0	50.0	58.3	57.9	548-553 bp	1.0	78.7