Hotspot

rpl32-trnL-UAG

SSC · 115505-116413 · 909 bp

Back to Aulacospermum

Pi 0.107

MarkerSeek score 83.60

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 909
variable_sites: 92
pi: 0.107
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGGCAACGGGTTCTTAAAGG	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.1	58.0	1335-1553 bp	1.0	89.7
rpl32-trnL-UAG_p2	AGGCAACGGGTTCTTAAAGG	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.1	58.0	1336-1554 bp	1.0	89.7
rpl32-trnL-UAG_p3	GGGTATTGGGCAGCCTTAAA	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.8	58.0	1034 bp	0.5	58.7
rpl32-trnL-UAG_p4	AGGCAACGGGTTCTTAAAGG	ACCAAAGGAAAAAGCCTCGT	50.0	45.0	58.1	57.9	2255 bp	0.5	58.7
rpl32-trnL-UAG_p5	GGGTATTGGGCAGCCTTAAA	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.8	58.0	1035 bp	0.5	58.7