Hotspot

rrn4.5-rrn5

IRb · 108393-108616 · 224 bp

Back to Aulacospermum

Pi 0.0938

MarkerSeek score 82.10

Primer available yes

Label rrn4.5-rrn5

Feature Metadata

label_name: rrn4.5-rrn5
feature_type: igs
region: IRb
length_bp: 224
variable_sites: 21
pi: 0.0938
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn4.5-rrn5_p1	CAGCTGAGGCATCCTAACAG	TATCGTCACCGCAGTAGAGT	55.0	50.0	58.0	58.0	339-373 bp	1.0	84.1
rrn4.5-rrn5_p2	CAGCTGAGGCATCCTAACAG	AGTATCGTCACCGCAGTAGA	55.0	50.0	58.0	58.0	341-375 bp	1.0	84.0
rrn4.5-rrn5_p3	GCTGAGGCATCCTAACAGAC	TATCGTCACCGCAGTAGAGT	55.0	50.0	58.1	58.0	337-371 bp	1.0	84.0
rrn4.5-rrn5_p4	GTCAAGTGGAAGTGCAGTGA	TATCGTCACCGCAGTAGAGT	50.0	50.0	58.0	58.0	365-399 bp	1.0	83.8
rrn4.5-rrn5_p5	GTCAAGTGGAAGTGCAGTGA	AGTATCGTCACCGCAGTAGA	50.0	50.0	58.0	58.0	367-401 bp	1.0	83.7