Hotspot

petA-psbJ

LSC · 64351-65359 · 1009 bp

Back to Aulacospermum

Pi 0.07
MarkerSeek score 79.70
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1009
variable_sites
63
pi
0.07
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.89
markerseek_score
79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GAGAAGGTTCAATTGTCAGA GCTGGTATTCTTGTGATCGGT 40.0 47.6 52.8 58.1 1028-1117 bp 1.0 48.7
petA-psbJ_p2 GAGAAGGTTCAATTGTCAGAAATGA GCTGGTATTCTTGTGATCGGT 36.0 47.6 57.8 58.1 1028-1117 bp 1.0 48.7
petA-psbJ_p3 AGAAGGTTCAATTGTCAGAAATGA GCTGGTATTCTTGTGATCGGT 33.3 47.6 56.8 58.1 1027-1116 bp 1.0 48.7
petA-psbJ_p4 GAGAAGGTTCAATTGTCAGAA GCTGGTATTCTTGTGATCGGT 38.1 47.6 53.8 58.1 1028-1117 bp 1.0 48.7
petA-psbJ_p5 GAGAAGGTTCAATTGTCAGA AGCTGGTATTCTTGTGATCGG 40.0 47.6 52.8 57.8 1029-1118 bp 1.0 48.7