Hotspot

ndhC-trnV-UAC

LSC · 51828-52505 · 678 bp

Back to Aulacospermum

Pi 0.0828
MarkerSeek score 83.20
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
678
variable_sites
52
pi
0.0828
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 AGATCATATTCGTAAAGCAGAAACA CCGAGAAAGTCTACGGTTCG 32.0 55.0 56.9 58.1 740-1126 bp 1.0 48.5
ndhC-trnV-UAC_p2 TCATATTCGTAAAGCAGAAACA CCGAGAAAGTCTACGGTTCG 31.8 55.0 53.9 58.1 737-1123 bp 1.0 48.5
ndhC-trnV-UAC_p3 TGCCAAAATAGGAATAAGACTTGA CCGAGAAAGTCTACGGTTCG 33.3 55.0 56.3 58.1 786-1172 bp 1.0 48.2
ndhC-trnV-UAC_p4 TGCCAAAATAGGAATAAGACTTG CCGAGAAAGTCTACGGTTCG 34.8 55.0 54.9 58.1 786-1172 bp 1.0 48.2
ndhC-trnV-UAC_p5 TGCCAAAATAGGAATAAGACTTGAT CCGAGAAAGTCTACGGTTCG 32.0 55.0 56.8 58.1 786-1172 bp 1.0 48.2