Hotspot

trnS-UGA-psbZ

LSC · 36734-37102 · 369 bp

Back to Aulacospermum

Pi 0.0745

MarkerSeek score 82.40

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 369
variable_sites: 26
pi: 0.0745
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAACCACTCAGCCATCTCTC	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.0	57.8	469-489 bp	1.0	85.9
trnS-UGA-psbZ_p2	GAGCTATCAACCACTCAGCC	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.1	57.8	476-496 bp	1.0	85.5
trnS-UGA-psbZ_p3	GGAGCTATCAACCACTCAGC	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.1	57.8	477-497 bp	1.0	85.5
trnS-UGA-psbZ_p4	CAACCACTCAGCCATCTCTC	GACCAACCATCAGGAGAAGC	55.0	55.0	58.0	58.3	471-491 bp	1.0	85.4
trnS-UGA-psbZ_p5	CAAGACCGGAGCTATCAACC	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.1	57.8	484-504 bp	1.0	85.3