Hotspot

petN-psbM

LSC · 29321-30432 · 1112 bp

Back to Aulacospermum

Pi 0.0834

MarkerSeek score 79.40

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1112
variable_sites: 90
pi: 0.0834
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	CGTCGTATGGGGAAGAAGTG	TCCCCTTCTTACTCCGGTTT	55.0	50.0	58.1	58.0	1588-1612 bp	1.0	86.6
petN-psbM_p2	GCTATCACGACTTCCCGATC	TCCCCTTCTTACTCCGGTTT	55.0	50.0	57.9	58.0	2103 bp	0.5	59.3
petN-psbM_p3	GCTATCACGACTTCCCGATC	TTTTCGTTTCGCCCTTCTCA	55.0	45.0	57.9	58.0	2379 bp	0.5	59.2
petN-psbM_p4	GCTATCACGACTTCCCGATC	CTTTCCCTTTTCGTTTCGCC	55.0	50.0	57.9	58.0	2386 bp	0.5	59.2
petN-psbM_p5	GACTTCCCGATCCCACTCTA	TCCCCTTCTTACTCCGGTTT	55.0	50.0	57.9	58.0	2095 bp	0.5	59.2