Hotspot

rpoB

LSC · 24923-28135 · 3213 bp

Pi 0.00323

MarkerSeek score 50.20

Primer available yes

Label rpoB

Feature Metadata

label_name: rpoB
feature_type: gene
region: LSC
length_bp: 3213
variable_sites: 25
pi: 0.00323
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB_p1	CTGCTAACCCGAGTGAACAT	CCTATAGGTGGTAGGGGTCG	50.0	60.0	57.6	58.1	292 bp	1.0	77.4
rpoB_p2	CTGCTAACCCGAGTGAACAT	CTATAGGTGGTAGGGGTCGG	50.0	60.0	57.6	58.1	291 bp	1.0	77.4
rpoB_p3	CTGCTAACCCGAGTGAACAT	GGGGTCGGGTTATTGATGTG	50.0	55.0	57.6	58.3	279 bp	1.0	75.5
rpoB_p4	CTGCTAACCCGAGTGAACAT	AGGGGTCGGGTTATTGATGT	50.0	50.0	57.6	58.4	280 bp	1.0	74.8
rpoB_p5	CTGCTAACCCGAGTGAACAT	GGTAGGGGTCGGGTTATTGA	50.0	55.0	57.6	58.5	283 bp	1.0	74.0