Hotspot

ccsA

SSC · 117966-118931 · 966 bp

Pi 0.00761

MarkerSeek score 49.10

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 966
variable_sites: 16
pi: 0.00761
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TCCATTCTGTAATTAAGGGACTCC	GGTACTCCGTGTTGTATTCGT	41.7	47.6	57.8	57.8	1056 bp	1.0	41.4
ccsA_p2	TCCATTCTGTAATTAAGGGACTCC	AGGTACTCCGTGTTGTATTCG	41.7	47.6	57.8	57.5	1057 bp	1.0	41.4
ccsA_p3	TCAATTCACGATTTCCATTCTGT	GGTACTCCGTGTTGTATTCGT	34.8	47.6	56.8	57.8	1069 bp	1.0	41.3
ccsA_p4	TCAATTCACGATTTCCATTCTGT	AGGTACTCCGTGTTGTATTCG	34.8	47.6	56.8	57.5	1070 bp	1.0	41.3
ccsA_p5	TTCAATTCACGATTTCCATTCTGT	GGTACTCCGTGTTGTATTCGT	33.3	47.6	57.4	57.8	1070 bp	1.0	41.3