Hotspot

ccsA-ndhD

SSC · 118932-119166 · 235 bp

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Pi 0.0273
MarkerSeek score 43.20
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
235
variable_sites
14
pi
0.0273
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
43.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 GGTGCAAATTCCGCAATTGT TCAGTAGCTTTTCGATGGCC 45.0 50.0 58.2 58.0 729 bp 1.0 80.5
ccsA-ndhD_p2 AGGCTTTGTTACTTCGGGTC TCAGTAGCTTTTCGATGGCC 50.0 50.0 57.8 58.0 1243 bp 1.0 80.2
ccsA-ndhD_p3 GGCTTTGTTACTTCGGGTCT TCAGTAGCTTTTCGATGGCC 50.0 50.0 57.8 58.0 1242 bp 1.0 80.2
ccsA-ndhD_p4 AGGCTTTGTTACTTCGGGTC CAGTAGCTTTTCGATGGCCT 50.0 50.0 57.8 58.0 1242 bp 1.0 80.1
ccsA-ndhD_p5 GGCTTTGTTACTTCGGGTCT CAGTAGCTTTTCGATGGCCT 50.0 50.0 57.8 58.0 1241 bp 1.0 80.1