Hotspot

psbE-petL

LSC · 67798-69005 · 1208 bp

Pi 0.00605

MarkerSeek score 51.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1208
variable_sites: 21
pi: 0.00605
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGTCGTATTTTGCTCAGACCA	50.0	42.9	57.9	57.3	1401-1416 bp	1.0	67.4
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	AGTCGTATTTTGCTCAGACCA	50.0	42.9	57.8	57.3	1324-1339 bp	1.0	66.6
psbE-petL_p3	ACGAATAACCAACCCGCAAT	AGTCGTATTTTGCTCAGACCA	45.0	42.9	58.2	57.3	1410-1425 bp	1.0	66.4
psbE-petL_p4	TGCTGACGAATAACCAACCC	AGTCGTATTTTGCTCAGACCA	50.0	42.9	58.2	57.3	1415-1430 bp	1.0	66.3
psbE-petL_p5	CGAATAACCAACCCGCAATG	AGTCGTATTTTGCTCAGACCA	50.0	42.9	57.8	57.3	1409-1424 bp	1.0	66.3