Hotspot

ycf4-cemA

LSC · 64010-64350 · 341 bp

Back to Aucuba

Pi 0.0157
MarkerSeek score 42.20
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
341
variable_sites
11
pi
0.0157
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
42.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 TTGGCCTATTTCTTGCGTGT AGAGGGGTGAATGCTTTCTT 45.0 45.0 58.1 56.7 407-428 bp 1.0 71.5
ycf4-cemA_p2 TTGGCCTATTTCTTGCGTGT AAGAGGGGTGAATGCTTTCT 45.0 45.0 58.1 56.7 408-429 bp 1.0 71.4
ycf4-cemA_p3 AAAAAGCTGCTGAATTGGCC AAGAGGGGTGAATGCTTTCT 45.0 45.0 57.8 56.7 422-443 bp 1.0 70.7
ycf4-cemA_p4 AAAAAGCTGCTGAATTGGCC AGAGGGGTGAATGCTTTCTT 45.0 45.0 57.8 56.7 421-442 bp 1.0 70.7
ycf4-cemA_p5 AAAGCTGCTGAATTGGCCTA AGAGGGGTGAATGCTTTCTT 45.0 45.0 57.8 56.7 419-440 bp 1.0 70.4