Hotspot

accD-psaI

LSC · 62202-62896 · 695 bp

Back to Aucuba

Pi 0.0106
MarkerSeek score 42.10
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
695
variable_sites
17
pi
0.0106
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
42.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 CTCCACGCCTTCTTTCCTTT CCATTGCAATTGCCGGAAAT 50.0 45.0 58.1 58.0 787-801 bp 1.0 81.8
accD-psaI_p2 TACTACTGGTGGGGTGACAG CCATTGCAATTGCCGGAAAT 55.0 45.0 58.1 58.0 1010-1024 bp 1.0 81.5
accD-psaI_p3 CTACTACTGGTGGGGTGACA CCATTGCAATTGCCGGAAAT 55.0 45.0 58.1 58.0 1011-1025 bp 1.0 81.4
accD-psaI_p4 CTACTACTGGTGGGGTGACA ATTTCAAGCCATCCAGCGAT 55.0 45.0 58.1 57.9 1269-1283 bp 1.0 81.1
accD-psaI_p5 TACTACTGGTGGGGTGACAG ATTTCAAGCCATCCAGCGAT 55.0 45.0 58.1 57.9 1268-1282 bp 1.0 81.1