Hotspot

trnE-UUC-trnT-GGU

LSC · 33122-33991 · 870 bp

Back to Aucuba

Pi 0.00495
MarkerSeek score 49.00
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
870
variable_sites
12
pi
0.00495
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.97
markerseek_score
49.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 TTTCGTAGTACCCTACCCCC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 987-1011 bp 1.0 81.6
trnE-UUC-trnT-GGU_p2 TTTCGTAGTACCCTACCCCC CCATGGCGTTACTCTACCAC 55.0 55.0 57.8 58.1 966-990 bp 1.0 81.2
trnE-UUC-trnT-GGU_p3 TTTCGTAGTACCCTACCCCC TGGCGTTACTCTACCACTGA 55.0 50.0 57.8 58.1 963-987 bp 1.0 81.0
trnE-UUC-trnT-GGU_p4 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.3 58.0 949-973 bp 1.0 80.9
trnE-UUC-trnT-GGU_p5 TGTCCTGAACCACTAGACGA GAACCGATGACTTACGCCTT 50.0 50.0 57.7 58.0 928-952 bp 1.0 80.8