Hotspot

trnC-GCA-petN

LSC · 29474-30173 · 700 bp

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Pi 0.0148
MarkerSeek score 50.90
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
700
variable_sites
23
pi
0.0148
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTCCCCAGTTCAAATCCGG ACTTCTTCCCCACACTACGA 50.0 50.0 58.1 58.0 810-940 bp 1.0 81.4
trnC-GCA-petN_p2 TTTCCCCAGTTCAAATCCGG TTCTTCCCCACACTACGAGT 50.0 50.0 58.1 58.0 808-938 bp 1.0 81.4
trnC-GCA-petN_p3 TTTCCCCAGTTCAAATCCGG CACTTCTTCCCCACACTACG 50.0 55.0 58.1 57.9 811-941 bp 1.0 80.8
trnC-GCA-petN_p4 TTTCCCCAGTTCAAATCCGG TCTAGAGCCCACTTCTTCCC 50.0 55.0 58.1 58.1 820-950 bp 1.0 80.4
trnC-GCA-petN_p5 TTTCCCCAGTTCAAATCCGG AGCCCAAGCGAGACTTACTA 50.0 50.0 58.1 58.2 756-886 bp 1.0 80.4