Hotspot

rpl16

LSC · 81579-83169 · 1591 bp

Back to Attalea

Pi 0.00168

MarkerSeek score 62.40

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1591
variable_sites: 4
pi: 0.00168
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCAATGCAAAGGATGAAGGA	AGGTTGAATAAAAATTCGATTGACCA	40.0	30.8	54.9	58.1	1707-1738 bp	1.0	41.9
rpl16_p2	TCAATGCAAAGGATGAAGGA	GGTTGAATAAAAATTCGATTGACCA	40.0	32.0	54.9	56.9	1706-1737 bp	1.0	41.9
rpl16_p3	TCAATGCAAAGGATGAAGGAA	AGGTTGAATAAAAATTCGATTGACCA	38.1	30.8	55.7	58.1	1707-1738 bp	1.0	41.9
rpl16_p4	TTCAATGCAAAGGATGAAGGA	AGGTTGAATAAAAATTCGATTGACCA	38.1	30.8	55.7	58.1	1708-1739 bp	1.0	41.9
rpl16_p5	TCAATGCAAAGGATGAAGGAA	GGTTGAATAAAAATTCGATTGACCA	38.1	32.0	55.7	56.9	1706-1737 bp	1.0	41.9