Hotspot

ndhB-trnL-CAA

IRa · 144116-144697 · 582 bp

Back to Attalea

Pi 0.0592
MarkerSeek score 68.40
Primer available yes
Label ndhB-trnL-CAA

Feature Metadata

label_name
ndhB-trnL-CAA
feature_type
igs
region
IRa
length_bp
582
variable_sites
49
pi
0.0592
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhB-trnL-CAA_p1 TTGCGATTGCTCAGGATACC GTCTCGCGTGTCTACCATTT 50.0 50.0 58.0 58.0 624-649 bp 1.0 90.8
ndhB-trnL-CAA_p2 ACCCGATTATTGCGATTGCT GTCTCGCGTGTCTACCATTT 45.0 50.0 58.0 58.0 658 bp 0.333333 53.0
ndhB-trnL-CAA_p3 ACCCGATTATTGCGATTGCT CTCGCGTGTCTACCATTTCA 45.0 50.0 58.0 58.0 656 bp 0.333333 53.0
ndhB-trnL-CAA_p4 TTATTCTGGTGTGGATGGCG GTCTCGCGTGTCTACCATTT 50.0 50.0 58.0 58.0 896 bp 0.333333 53.0
ndhB-trnL-CAA_p5 TTATTCTGGTGTGGATGGCG CTCGCGTGTCTACCATTTCA 50.0 50.0 58.0 58.0 894 bp 0.333333 53.0