Hotspot

psaC-ndhE

SSC · 117568-118213 · 646 bp

Back to Attalea

Pi 0.00313

MarkerSeek score 62.10

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 646
variable_sites: 3
pi: 0.00313
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	ACATCCGTCCCAAGGTATCA	TGAAGCAGCTATTGGGCTAG	50.0	50.0	58.1	57.4	800-839 bp	1.0	78.1
psaC-ndhE_p2	ACATCCGTCCCAAGGTATCA	CTGAAGCAGCTATTGGGCTA	50.0	50.0	58.1	57.4	801-840 bp	1.0	78.0
psaC-ndhE_p3	ACATCCGTCCCAAGGTATCA	TGTTATAGCTGTTGCGGCTG	50.0	50.0	58.1	58.6	821-860 bp	1.0	77.9
psaC-ndhE_p4	ACATCCGTCCCAAGGTATCA	GAAGCAGCTATTGGGCTAGC	50.0	55.0	58.1	58.8	799-838 bp	1.0	76.9
psaC-ndhE_p5	CAATACATCTGTGGGGCAGG	TGAAGCAGCTATTGGGCTAG	55.0	50.0	58.3	57.4	776-815 bp	1.0	76.7