Hotspot

rpl32-trnL-UAG

SSC · 113742-114356 · 615 bp

Back to Attalea

Pi 0.0163

MarkerSeek score 69.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 615
variable_sites: 15
pi: 0.0163
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGTTTCTACCGGACATTCAA	AGCGTGTCTACCGATTTCAC	45.0	50.0	55.4	58.0	707-708 bp	1.0	60.8
rpl32-trnL-UAG_p2	GGTTTCTACCGGACATTCAA	CAGCGTGTCTACCGATTTCA	45.0	50.0	55.4	58.0	708-709 bp	1.0	60.8
rpl32-trnL-UAG_p3	GGTTTCTACCGGACATTCAA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	55.4	57.9	717-718 bp	1.0	59.8
rpl32-trnL-UAG_p4	GGTTTCTACCGGACATTCAA	CCTAAGAGCAGCGTGTCTAC	45.0	55.0	55.4	57.8	716-717 bp	1.0	59.2
rpl32-trnL-UAG_p5	GGTTTCTACCGGACATTCAA	CTAAGAGCAGCGTGTCTACC	45.0	55.0	55.4	57.8	715-716 bp	1.0	59.2