Hotspot

trnL-CAA-ndhB

IRb · 95015-95596 · 582 bp

Back to Attalea

Pi 0.0592

MarkerSeek score 68.40

Primer available yes

Label trnL-CAA-ndhB

Feature Metadata

label_name: trnL-CAA-ndhB
feature_type: igs
region: IRb
length_bp: 582
variable_sites: 49
pi: 0.0592
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ndhB_p1	GTCTCGCGTGTCTACCATTT	TTGCGATTGCTCAGGATACC	50.0	50.0	58.0	58.0	624-649 bp	1.0	90.8
trnL-CAA-ndhB_p2	GTCTCGCGTGTCTACCATTT	ACCCGATTATTGCGATTGCT	50.0	45.0	58.0	58.0	658 bp	0.333333	53.0
trnL-CAA-ndhB_p3	CTCGCGTGTCTACCATTTCA	ACCCGATTATTGCGATTGCT	50.0	45.0	58.0	58.0	656 bp	0.333333	53.0
trnL-CAA-ndhB_p4	GTCTCGCGTGTCTACCATTT	TTATTCTGGTGTGGATGGCG	50.0	50.0	58.0	58.0	896 bp	0.333333	53.0
trnL-CAA-ndhB_p5	CTCGCGTGTCTACCATTTCA	TTATTCTGGTGTGGATGGCG	50.0	50.0	58.0	58.0	894 bp	0.333333	53.0