Hotspot

ndhF-rpl32

SSC · 111130-112082 · 953 bp

Back to Atriplex

Pi 0.0206

MarkerSeek score 60.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 953
variable_sites: 38
pi: 0.0206
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TAGGGCGTGTGAACCAAAAA	AAGTCGCGAACACTTTTCCT	45.0	45.0	57.9	58.1	1956-1992 bp	1.0	83.3
ndhF-rpl32_p2	GAAGAGGAAATTGGGCCGAT	AAGTCGCGAACACTTTTCCT	50.0	45.0	57.9	58.1	2187-2223 bp	1.0	83.0
ndhF-rpl32_p3	TAGGGCGTGTGAACCAAAAA	TTAATGCCGCCCAATATCCC	45.0	50.0	57.9	58.1	1544-1549 bp	1.0	81.6
ndhF-rpl32_p4	CGAAACTCGAAACTACCCGT	AAGTCGCGAACACTTTTCCT	50.0	45.0	57.9	58.1	2096-2101 bp	0.6	63.7
ndhF-rpl32_p5	CGAAACTCGAAACTACCCGT	TTAATGCCGCCCAATATCCC	50.0	50.0	57.9	58.1	1657-1658 bp	0.6	63.3