Hotspot

trnT-UGU-trnL-UAA

LSC · 46513-47279 · 767 bp

Back to Atriplex

Pi 0.0136

MarkerSeek score 60.00

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 767
variable_sites: 21
pi: 0.0136
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	AGCCAATTAAGTCCGTAGCG	55.0	50.0	57.9	58.1	840-848 bp	1.0	80.9
trnT-UGU-trnL-UAA_p2	GACTCGAACCGATGACCATC	AGCCAATTAAGTCCGTAGCG	55.0	50.0	58.2	58.1	871-879 bp	1.0	80.6
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	828-836 bp	1.0	79.5
trnT-UGU-trnL-UAA_p4	GACTCGAACCGATGACCATC	CCGTAGCGTCTACCGATTTC	55.0	55.0	58.2	58.2	859-867 bp	1.0	79.2
trnT-UGU-trnL-UAA_p5	GCGATGCTCTAACCTCTGAG	GCCAATTAAGTCCGTAGCGT	55.0	50.0	57.9	58.4	839-847 bp	1.0	78.6