Hotspot

ycf3-trnS-GGA

LSC · 44039-44986 · 948 bp

Back to Atriplex

Pi 0.0175

MarkerSeek score 58.30

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 948
variable_sites: 33
pi: 0.0175
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	ACTTCACCTTCAACAAGCGT	TGGCGAGGGATGAACAAAAA	45.0	45.0	58.0	58.0	1840-1863 bp	1.0	82.5
ycf3-trnS-GGA_p2	ACTTCACCTTCAACAAGCGT	GGAGCCCGCCAATTAGTTAA	45.0	50.0	58.0	58.0	1934-1957 bp	1.0	82.1
ycf3-trnS-GGA_p3	ACTTCACCTTCAACAAGCGT	TTTGCCCCAGATTTTCCCTC	45.0	50.0	58.0	58.1	1554-1577 bp	1.0	82.1
ycf3-trnS-GGA_p4	ACTTCACCTTCAACAAGCGT	ATCGTTGCAAACCCAAGGAT	45.0	45.0	58.0	58.1	1867-1890 bp	1.0	81.9
ycf3-trnS-GGA_p5	CCTATGCCGCCTATTGGTAC	TGGCGAGGGATGAACAAAAA	55.0	45.0	57.9	58.0	2005-2028 bp	1.0	81.8